Pro-angiogenic efficacy of transplanting endothelial progenitor cells for treating hindlimb ischemia in hyperglycemic rabbits

被引:11
|
作者
Yu, Ping [1 ]
Li, Qiang [1 ]
Liu, Ying [3 ]
Zhang, Jinchao [1 ]
Seldeen, Ken [2 ]
Pang, Manhui [2 ]
机构
[1] Harbin Med Univ, Affiliated Hosp 2, Dept Endocrinol & Metab, Harbin 150086, Peoples R China
[2] Miami Vet Affairs Healthcare Syst, Geriatr Res Educ & Clin Ctr, Miami, FL 33125 USA
[3] Harbin Med Univ, Affiliated Hosp 5, Daqing Peoples Hosp, Daqing 163316, Peoples R China
基金
黑龙江省自然科学基金;
关键词
EPCs; Hindlimb ischemia; Transplantation; Hyperglycemia; Neovascularization; BONE-MARROW; MEDIATED ANGIOGENESIS; DIABETES-MELLITUS; MYOCARDIAL REPAIR; SKELETAL-MUSCLES; THERAPY; DISEASE; MOBILIZATION; EXPRESSION; MOUSE;
D O I
10.1016/j.jdiacomp.2014.09.003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aims: To evaluate the effectiveness of endothelial progenitor cells (EPCs) therapy in ischemia with or without hyperglycemia. Methods: Japanese White Rabbits were randomly assigned to three groups, group SH, hyperglycemia with sham therapy (n = 10); group NE, normoglycemia with autologous EPCs transplantation therapy (n = 12); and group HE, hyperglycemia with autologous EPCs transplantation therapy (n = 12). Hyperglycemia was induced by injecting alloxan and sustained for 12 weeks. Hindlimb ischemia was induced by complete excision of the femoral artery. Ex vivo-expanded EPCs were derived from autologous bone marrow and transplanted intermuscularily in the ischemic hindlimb. Fourteen days after transplantation, the indicators were determined. Results: There is no difference of the functions of ex vivo-expanded EPCs from autologous bone marrow between normoglycemic and hyperglycemic groups. We found significant improvement in both EPCs transplantation therapy groups compared to sham, in terms of the angiogenesis index (8.62 +/- 1.36, 11.12 +/- 2.23, 12.35 +/- 2.97), capillary density (7.06 +/- 0.91, 13.51 +/- 1.16, 13.90 +/- 2.78), capillary to muscle fiber ratio (0.68 +/- 0.09, 0.96 +/- 0.11,0.89 +/- 0.10), muscle VEGF expression (0.22 +/- 0.07, 0.41 +/- 0.08, 0.38 +/- 0.07 ng/g). We found no significant differences between hyperglycemic and normoglycemic EPCs therapy groups except for 5 pro-angiogenic genes that were upregulated in HE as compared to NE. Conclusion: Ex vivo expanded EPCs from autologous bone marrow transplantation is an effective therapeutic method for hindlimb ischemia in rabbits regardless of glycemic state. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:13 / 19
页数:7
相关论文
共 50 条
  • [1] Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells
    Jabarpour, Masoumeh
    Siavashi, Vahid
    Asadian, Simin
    Babaei, Homa
    Jafari, Seyedeh Maedeh
    Nassiri, Seyed Mandi
    [J]. MICROVASCULAR RESEARCH, 2018, 118 : 49 - 56
  • [2] Epigenetic Regulation of Endothelial Lineage Committed Genes in Pro-Angiogenic Hematopoietic and Endothelial Progenitor Cells
    Ohtani, Kisho
    Vlachojannis, Georgios J.
    Koyanagi, Masamichi
    Boeckel, Jes-Niels
    Urbich, Carmen
    Farcas, Ruxandra
    Bonig, Halvard
    Marquez, Victor E.
    Zeiher, Andreas M.
    Dimmeler, Stefanie
    [J]. CIRCULATION RESEARCH, 2011, 109 (11) : 1219 - U71
  • [3] Homeobox gene expression induces a pro-angiogenic state following hindlimb ischemia
    Lane, JS
    Chan, T
    Messina, L
    Boudreau, N
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS, 2004, 199 (03) : S110 - S110
  • [4] Endothelial progenitor cells give rise to pro-angiogenic smooth muscle-like progeny
    Moonen, Jan-Renier A. J.
    Krenning, Guido
    Brinker, Marja G. L.
    Koerts, Jasper A.
    van Luyn, Marja J. A.
    Harmsen, Martin C.
    [J]. CARDIOVASCULAR RESEARCH, 2010, 86 (03) : 506 - 515
  • [5] Pro-angiogenic properties are impaired in bone marrow (BM)-derived stem or progenitor cells in diabetes and diabetic hindlimb ischemia was effectively repaired by non-diabetic stem or progenitor cells
    Lee, Juyong
    Lee, Mejeong
    Lee, Jiyoon
    Silver, Marcy
    Burg, Aaron
    Mehta, Shanu
    Wecker, Andrea
    Jeong, Jin-Ok
    Cho, Hyun-Jai
    Cho, Yong-Pil
    Yoon, Young-sup
    [J]. CIRCULATION, 2006, 114 (18) : 103 - 103
  • [6] Inflammation is associated with a reduced number of pro-angiogenic Tie-2 monocytes and endothelial progenitor cells in patients with critical limb ischemia
    Jörn F. Dopheide
    Philipp Geissler
    Jennifer Rubrech
    Amelie Trumpp
    Geraldine C. Zeller
    Karsten Bock
    Bernhard Dorweiler
    Friedrich Dünschede
    Thomas Münzel
    Markus P. Radsak
    Christine Espinola-Klein
    [J]. Angiogenesis, 2016, 19 : 67 - 78
  • [7] Inflammation is associated with a reduced number of pro-angiogenic Tie-2 monocytes and endothelial progenitor cells in patients with critical limb ischemia
    Dopheide, Joern F.
    Geissler, Philipp
    Rubrech, Jennifer
    Trumpp, Amelie
    Zeller, Geraldine C.
    Bock, Karsten
    Dorweiler, Bernhard
    Duenschede, Friedrich
    Muenzel, Thomas
    Radsak, Markus P.
    Espinola-Klein, Christine
    [J]. ANGIOGENESIS, 2016, 19 (01) : 67 - 78
  • [8] CTACK a new pro-angiogenic factor for dermal endothelial cells
    Franchi, J.
    Crola, C.
    Lardin, C.
    Marteau, C.
    Mitterrand, M.
    Schnebert, S.
    Kieda, C.
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2006, 126 : 60 - 60
  • [9] Effect of antiviral therapy on pro-angiogenic hematopoietic and endothelial progenitor cells in HIV-infected people
    Vecchiet, Jacopo
    Iachininoto, Maria Grazia
    Capodimonti, Sara
    Nuzzolo, Eugenia Rosa
    Falasca, Katia
    Martini, Maurizio
    Mancino, Paola
    Bianchi, Maria
    Leone, Antonio Maria
    Ucciferri, Claudio
    Larocca, Luigi Maria
    Teofili, Luciana
    [J]. THROMBOSIS RESEARCH, 2013, 131 (03) : 238 - 243
  • [10] HMGB-1 induces pro-angiogenic effects in endothelial cells: Implications for lower extremity ischemia
    Sachdev, Ulka
    Tzeng, Edith
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS, 2007, 205 (03) : S108 - S109