Development of ancillary techniques in capillary supercritical fluid chromatography

Capillary supercritical fluid chromatography (SFC) has been recognized as being a powerful tool for the separation of thermally labile or involatile species which are not amenable to gas chromatography. It also provides a higher column efficiency than does HPLC. To fully utilize the ability and high efficiency of capillary SFC, however, further improvements in the current technology and the development of new techniques are indispensable. First, in this article, various sample-introduction methods which can inject sample volumes ranging from low to high micro litters are presented. Second, methods for controlling the UV cell pressure and temperature as well as preparing a mixed mobile phase are presented, and their ability demonstrated. Finally, an interface for two-dimensional capillary SFC and its principle are described.