Down regulation of miR-18a, miR-21 and miR-221 genes in gastric cancer cell line by chrysin-loaded PLGA-PEG nanoparticles

被引：45
|
作者：
Mohammadian, Farideh [1 ,2 ,3 ]
Pilehvar-Soltanahmadi, Yones [1 ]
Mofarrah, Mohsen [1 ]
Dastani-Habashi, Mehdi [1 ]
Zarghami, Nosratollah [1 ,2 ]
机构：
[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Med Biotechnol, Tabriz, Iran
[2] Tabriz Univ Med Sci, Hematol & Oncol Res Ctr, Tabriz, Iran
[3] Tabriz Univ Med Sci, Int Aras Branch, Tabriz, Iran
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2016年 / 44卷 / 08期
关键词：
Chrysin; miRNAs; PLGA-PEG; real-time PCR; BODY-MASS INDEX; TUMOR-SUPPRESSOR; RISK;
D O I：
10.3109/21691401.2015.1129615
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Chrysin were well-documented as having significant biological roles particularly cancer chemo-preventive activity. However, the poor water solubility of chrysin limited their bioavailability and biomedical applications. In this study, we encapsulate the chrysin into PLGA-PEG nanoparticles for local treatment. In regard to the amount of the drug load, IC50 was significant decreased in nanocapsulated chrysin in comparison with free chrysin. This was confirmed through decrease of miR-18a, miR-21, and miR-221 genes expression by real-time PCR. The results demonstrated that PLGA-PEG-chrysin complexes can be more effective than free chrysin. Therefore, PLGA-PEG can be a better nanocarrier for this kind of hydrophobic flavonoid.
引用
收藏
页码：1972 / 1978
页数：7
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