Effect of a genetic variant in the JAZF1 gene among obesity population

Introduction: Obesity is a complex condition characterized by an excess of adipose tissue and a low-grade chronic inflammation. Juxtaposed with another zinc finger protein 1 (JAZF1) is one of the genes identified through genome wide association studies which is associated in type 2 diabetes, obesity and other forms of human diseases. JAZF1 controls the expression of several genes involved in lipid and carbohydrate metabolism. Limited studies have been conducted in the obesity population and none of the studies was documented in the Saudi population. The aim of this study was to investigate the genetic association with rs864745 SNP genotype assay in JAZF1 gene in the obesity population in Saudi Arabia. Methods: This study includes 80 obese patients and 50 healthy controls. Polymerase chain reaction was performed in the rs864745 SNP of JAZF1 gene using isolated genomic DNA from 130 participants. Anthropometric measurements were calculated between cases and controls using t-tests. HardyWeinberg Equilibrium (HWE) analysis, genotype and allele frequencies were calculated between obesity cases and controls. Anova analysis was carried out between rs864745 SNP genotype assay and anthropmetric measurements. Statistical analysis was performed using with SPSS software. Results: Weight, BMI, HDLC, LDLC, and TG were all associated with obesity in the clinical data (p < 0.05). The deviation with the rs864745 SNP genotyping assay was identified by HWE analysis. None of the genotypes, dominant models, or allelic frequencies demonstrated a significant connection with obesity (p > 0.05). ANOVA analysis was not associated (p < 0.05) between genotypes and anthropometric parameters.Conclusion: In conclusion, rs864745 SNP genotype assay of the JAZF1 gene did not indicate a genetic association. Anova analysis also revealed the negative association. Future research with a large sample sizes are recommended.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).