Assessing the Morphological and Behavioral Toxicity of Catechol Using Larval Zebrafish

被引:2
|
作者
Morash, Michael G. [1 ]
Soanes, Kelly H. [2 ]
Achenbach, John C. [1 ]
Ellis, Lee D. [1 ]
机构
[1] Natl Res Council Canada, Aquat Crop & Resource Dev, Halifax, NS B3H 3Z1, Canada
[2] Natl Res Council Canada, Aquat Crop & Resource Dev, Saskatoon, SK S7N 0W9, Canada
关键词
catechol; zebrafish; toxicity; gene expression; melanocytes; behavior; DNA-ADDUCTS; PHENOLIC-COMPOUNDS; IN-VITRO; MAINSTREAM; HORMONE; EXPRESSION; EMBRYOS; CELLS;
D O I
10.3390/ijms23147985
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Catechol is a ubiquitous chemical used in the manufacturing of fragrances, pharmaceuticals and flavorants. Environmental exposure occurs in a variety of ways through industrial processes, during pyrolysis and in effluent, yet despite its prevalence, there is limited information regarding its toxicity. While the genotoxicity and gastric carcinogenicity of catechol have been described in depth, toxicological studies have potentially overlooked a number of other effects relevant to humans. Here, we have made use of a general and behavioral larval zebrafish toxicity assay to describe previously unknown catechol-based toxicological phenomena. Behavioral testing revealed catechol-induced hypoactivity at concentrations an order of magnitude lower than observable endpoints. Catechol exposure also resulted in punctate melanocytes with concomitant decreases in the expression of pigment production and regulation markers mitfa, mc1r and tyr. Because catechol is converted into a number of toxic metabolites by tyrosinase, an enzyme found almost exclusively in melanocytes, an evaluation of the effects of catechol on these cells is critical to evaluating the safety of this chemical. This work provides insights into the toxic nature of catechol and highlights the benefits of the zebrafish larval testing platform in being able to dissect multiple aspects of toxicity with one model.
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页数:10
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