Total saponins extracted from Abrus cantoniensis Hance suppress hepatitis B virus replication in vitro and in rAAV8-1.3HBV transfected mice

被引：23

作者：

Yao, Xiangcao ^{[1
]}

Li, Zhanquan ^{[1
]}

Gong, Xiaomei ^{[1
]}

Fu, Xiang ^{[1
]}

Xiao, Xiao ^{[2
]}

He, Maolin ^{[1
]}

Huang, Baokang ^{[2
]}

Xu, Zhongyuan ^{[1
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Res Ctr Clin Pharmacol, Guangzhou 510515, Peoples R China

[2] Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2020年 / 249卷

基金：

中国国家自然科学基金;

关键词：

Abrus cantoniensis; Genechip; Hepatitis B virus; HepG2.2.15; cells; Immunohistochemistry; TRITERPENE SAPONINS; MOUSE MODEL; INFECTION; EXPRESSION; CHALLENGES; GUIDELINES; MANAGEMENT;

D O I：

10.1016/j.jep.2019.112366

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Hepatitis B, an infectious disease caused by hepatitis B virus (HBV), is still a serious problem affecting global public health. Abrus cantoniensis Hance (AC), a traditional Chinese medicinal herb, has been used as a folk medicine for treating hepatitis in China from ancient times. However, its active ingredients are still unclear. Aim of study: Our previous study indicated that saponins extracted from AC (ACS) were the active anti-HBV ingredients in AC. This study aimed to further investigate the anti-HBV effect of ACS in vitro and in vivo. Materials and methods: HepG2.2.15 cells which consecutively produce HBV DNA and HBV antigens were used for in vitro test, and C57BL/6 mice infected by a recombinant adeno-associated virus 8 vector carrying 1.3 copies of HBV genome (rAAV8-HBV1.3) were used for in vivo test. The histopathological changes and the immune indices were evaluated in mice model. Genechip was conducted to identify genes and pathways regulated by ACS in HepG2.2.15 cells. Results: In this study, we confirmed that ACS treatment prominently inhibited production of HBV DNA, Hepatitis Be Antigen (HBeAg), and Hepatitis B surface antigen (HBsAg) in HepG2.2.15 cells. ACS treatment also decreased serum HBsAg, HBeAg, and HBV DNA level in rAAV8-1.3HBV transfected mice, which is in accordance with the in vitro results. Moreover, HBV infection-induced liver inflammation was significantly relieved by ACS, which could be observed in H&E staining and immunohistochemistry of HBcAg. ACS treatment elevated IFN-gamma level in mice serum and increased CD4(+) T cell percentage in splenocytes. KEGG pathway analysis showed that phenylalanine metabolism pathway and tyrosine metabolism pathway were greatly regulated by ACS treatment. Conclusion: ACS exerted potent inhibitory effects on HBV replication both in vivo and in vitro, which may provide basis for its potential clinical usage.

引用

页数：8