Human metapneumovirus detection in patients with severe acute respiratory syndrome

被引:1
|
作者
Chan, PKS [1 ]
Tam, JS [1 ]
Lam, CW [1 ]
Chan, E [1 ]
Wu, A [1 ]
Li, CK [1 ]
Buckley, TA [1 ]
Ng, KC [1 ]
Joynt, GM [1 ]
Cheng, FWT [1 ]
To, KF [1 ]
Lee, N [1 ]
Hui, DSC [1 ]
Cheung, JLK [1 ]
Chu, I [1 ]
Liu, E [1 ]
Chung, SCA [1 ]
Sung, JJY [1 ]
机构
[1] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Fac Med, Shatin, Hong Kong, Peoples R China
关键词
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.
引用
收藏
页码:1058 / 1063
页数:6
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