Botanical micelle and its application for direct electrochemical biosensor

This paper is concerned about the entrapment of horseradish peroxidase (HRP) within botanical inositol hexakisphosphoric (IP6) micelles for the preparation of enzyme biosensor. The good affinity of IP6 micelles with the enzyme provides naturally biocompatible microenvironment for the enzyme immobilization, achieving the direct electron transfer between HRP and electrode surface. The resulting biosensor to H2O2 detection exhibits a low detection limit of 0.1 mu mol L-1 (S/N =3), a quick response time (3 s), and a long-term stability. The apparent Michaelis-Menten constant is quite tiny about 0.0016 mmol L-1. (C) 2010 Elsevier B.V. All rights reserved.