Lanthanide Metal-Organic Framework Nanoprobes for the In Vitro Detection of Cardiac Disease Markers

Acute myocardial infarction (AMI) is one of the leading causes of death around the world. An early and accurate diagnosis of AMI is critical to reduce the mortality rate. As an important cardiac biomarker, creatine kinase (CK) has been used in the clinical diagnosis of AMI. However, it still remains a great challenge to realize highly sensitive and selective CK detection in blood specimens. Herein, we have developed an ultrasensitive platform for the detection of CK activity based on time-resolved (TR) luminescent lanthanide metal organic framework nanoprobes (Eu QPTCA). Benefiting from the intense emission of lanthanide ions sensitized by the organic ligands and the eliminated short-lived autofluorescence by the TR technique, these nanoprobes enabled the homogeneous detection of CK activity with a limit of detection down to 1.0 U/L, which is about 1 order of magnitude improvement relative to that of the traditional methods. In addition, the Eu QPTCA nanoprobes showed superior selectivity and reliability toward the practical detection of CK activity in human serum, indicating the great significance of our method in the early diagnosis of AMI. We envision that the proposed bioassay strategy can be extended to the detection of other phosphorylation enzymes, paving a way for promising applications in clinical diagnostics.