Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover

被引：81
|
作者：
Chen, Qin [1 ]
Marshall, Megan N. [1 ]
Geib, Scott M. [2 ]
Tien, Ming [3 ]
Richard, Tom L. [1 ]
机构：
[1] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA
[2] ARS, USDA, Basin Agr Res Ctr, Hilo, HI 96720 USA
[3] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
来源：
BIORESOURCE TECHNOLOGY | 2012年 / 117卷
关键词：
Laccase; Delignification; TMAH-GC-MS; Enzymatic hydrolysis; Ensilage; IN-SITU; PRETREATMENT; DEGRADATION; ENZYMES; FERMENTATION; PRODUCTS; REMOVAL; PULP; WOOD;
D O I：
10.1016/j.biortech.2012.04.085
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations. (C) 2012 Published by Elsevier Ltd.
引用
收藏
页码：186 / 192
页数：7
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