Blood Meal Source Characterization Using Illumina Sequencing in the Chagas Disease Vector Rhodnius pallescens (Hemiptera: Reduviidae) in Panama

被引：30

作者：

Kieran, Troy J. ^{[1
]}

Gottdenker, Nicole L. ^{[2
]}

Varian, Christina P. ^{[2
]}

Saldana, Azael ^{[3
]}

Means, Nicolas ^{[4
]}

Owens, Darlisha ^{[5
]}

Calzada, Jose E. ^{[3
]}

Glenn, Travis C. ^{[1
]}

机构：

[1] Univ Georgia, Dept Environm Hlth Sci, Coll Publ Hlth, Athens, GA 30602 USA

[2] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA 30602 USA

[3] Inst Conmemorat Gorgas Estudios Salud, Dept Parasitol, Panama City, Panama

[4] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA

[5] Grambling State Univ, Dept Biol, Grambling, LA USA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2017年 / 54卷 / 06期

基金：

美国国家科学基金会;

关键词：

high-throughput DNA sequencing; PCR; blood meal identification; disease vector; triatominae; IDENTIFICATION; TRIATOMINAE; PALMS;

D O I：

10.1093/jme/tjx170

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Accurate blood meal identification is critical to understand hematophagous vector-host relationships. This study describes a customizable Next-Generation Sequencing (NGS) approach to identify blood meals from Rhodnius pallescens (Hemiptera: Reduviidae) triatomines using multiple barcoded primers and existing software to pick operational taxonomic units and match sequences for blood meal identification. We precisely identified all positive control samples using this method and further examined 74 wild-caught R. pallescens samples. With this novel blood meal identification method, we detected 13 vertebrate species in the blood meals, as well as single and multiple blood meals in individual bugs. Our results demonstrate the reliability and descriptive uses of our method.

引用

页码：1786 / 1789

页数：4

共 25 条

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[2] An updated catalog of lipocalins of the chagas disease vector Rhodnius prolixus (Hemiptera, Reduviidae)
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INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2022, 146
[3] Microsatellite markers from' the Chagas disease vector, Rhodnius prolixus (Hemiptera, Reduviidae), and their applicability to Rhodnius species
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INFECTION GENETICS AND EVOLUTION, 2008, 8 (03) : 381 - 385
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INFECTION GENETICS AND EVOLUTION, 2009, 9 (02) : 206 - 209
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Kieran, Troy J.
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Saldana, Azael
Samudio, Franklyn
Calzada, Jose E.
Gottdenker, Nicole L.
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INFECTION GENETICS AND EVOLUTION, 2020, 84
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OPEN BIOLOGY, 2021, 11 (06)
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