Qualitative and quantitative high performance liquid chromatographic analysis of quassinoids in Simaroubaceae plants

被引:0
|
作者
Dou, JH
Khan, IA
McChesney, JD
Burandt, CL
机构
[1] UNIV MISSISSIPPI, SCH PHARM, DEPT PHARMACOGNOSY, UNIVERSITY, MS 38677 USA
[2] UNIV MISSISSIPPI, SCH PHARM, PHARMACEUT SCI RES INST, UNIVERSITY, MS 38677 USA
关键词
quassinoids; Simaroubaceae; reversed phase high performance liquid chromatography;
D O I
10.1002/(SICI)1099-1565(199607)7:4<192::AID-PCA303>3.0.CO;2-Z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A convenient and reliable reversed phase high performance liquid chromatography method was developed using a phenyl column and gradient elution for the determination of eight important quassinoids including glaucarubolone, chaparrinone, holacanthone, glaucarubinone, picrasin B, paraine, neoquassin and quassin, in diverse tissues of species of the Simaroubaceae, The method gave well resolved peaks for these compounds with good response and linearity in the range of 25-1000 ng. Incorporation of an internal standard provided easy quantitation of the quassinoids.
引用
收藏
页码:192 / 200
页数:9
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