Application of aerobic granules-continuous flow reactor for saline wastewater treatment: Granular stability, lipid production and symbiotic relationship between bacteria and algae

In this study a continuous flow reactor (CFR) was employed to compare the feasibility of bacterial aerobic granular sludge (AGS-CFR) and algal-bacterial granular sludge (ABGS-CFR) for treating 1-4% saline wastewater. High salinity was found to enhance algae growth in ABGS-CFR, which exhibited slightly higher total nitrogen and phosphorus removal efficiencies at 1-3% salinity. ABGS-CFR maintained good granular stability at 1-4% salinity, while AGS-CFR gradually disintegrated at 4% salinity with 39.3% less accumulation of alginate-like exopolysaccharides in the extracellular polymeric substances. Indole-3-acetic acid (IAA) and superoxide dismutase (SOD) analysis suggested that bacteria and algae (Nitzschia) in ABGS-CFR formed a good symbiotic relationship under high salinity conditions, achieving rapid algae growth and 2 times lipid production. High salinity was conducive to enriching Halomonas and Nitzschia but unfavorable for Nitrosomonas and Flavobacterium. Results from this study could provide useful information on interactions between bacteria and algae in ABGS-CFR for its future practical application.