Screening of a phage display library of exendin-4 mutants with the extracellular domain of rat GLP-1 receptor

被引：0
|
作者：
Yin, Xiaopu [1 ]
Ma, Yushu [1 ]
Liu, Meiyun [1 ]
Gao, Weifeng [1 ]
Wei, Dongzhi [1 ]
机构：
[1] E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
PROTEIN AND PEPTIDE LETTERS | 2007年 / 14卷 / 08期
关键词：
exendin-4; GLP-1; receptor; phage display library;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A phage display library of exendin-4 mutants was screened with the extracellular domain of rat glucagon-like peptide 1 receptor as the target. A novel variant of exendin-4 with higher affinity for the receptor fraction than that of the wild type was identified. The increased affinity was attributed to the substitution of Glu 16 by Val 16. Although the substitution probably caused an increased entropic cost to the helix region, the linker around Val 16 is more flexible resulting in the increase of affinity for the receptor.
引用
收藏
页码：816 / 821
页数：6
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