Enzymatically triggered graphene oxide released from multifunctional carriers boosts anti-pathogenic properties for promising wound-healing applications

被引：26

作者：

Hieu Trung Nguyen ^{[1
,2
]}

Ho, Thi-Luu ^{[3
]}

Pratomo, Andi ^{[4
]}

Ilsan, Noor Andryan ^{[1
,5
]}

Huang, Tzu-Wen ^{[5
]}

Chen, Chih-Hwa ^{[3
,6
,7
]}

Chuang, Er-Yuan ^{[3
,8
]}

机构：

[1] Taipei Med Univ, Coll Med, Int PhD Program Med, Taipei 11031, Taiwan

[2] Univ Med & Pharm Ho Chi Minh City, Fac Med, Dept Orthoped & Trauma, Ho Chi Minh City, Vietnam

[3] Taipei Med Univ, Grad Inst Biomed Mat & Tissue Engn, Taipei 11031, Taiwan

[4] Taipei Med Univ, Coll Med, Int Master Program Med, Taipei 11031, Taiwan

[5] Taipei Med Univ, Coll Med, Sch Med, Dept Microbiol & Immunol, Taipei 11031, Taiwan

[6] Taipei Med Univ, Res Ctr Biomed Device, Taipei 11031, Taiwan

[7] Taipei Med Univ, Shuang Ho Hosp, Dept Orthoped, 291 Zhongzheng Rd, New Taipei 23561, Taiwan

[8] Taipei Med Univ, Wan Fang Hosp, Cell Physiol & Mol Image Res Ctr, 111,Sec 3,Xinglong 11 Rd, Taipei 11696, Taiwan

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 128卷

关键词：

Antibacterial; Wound healing; Crosslinking; Enzymatic degradation; Graphene oxide; Hydrogel; GENIPIN; GELATIN; SCAFFOLDS; MEMBRANE;

D O I：

10.1016/j.msec.2021.112265

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Spurred by recent progress in biomaterials and therapeutics, stimulus-responsive strategies that deliver an active substance in temporal-, spatial-, and dose-controlled fashions have become achievable. Implementation of such strategies necessitates the use of bio-safe materials that are sensitive to a specific pathological incitement or that, in response to a precise stimulus, undergo hydrolytic cleavage or a change in biomolecular conformation. An innovative design of polymeric stimulus-responsive systems should controllably release a drug or degrade the drug carrier in response to specific lesion enzymes. Wound healing is a great challenge due to various hidden factors such as pathogenic infections, neurovascular diseases, excessive exudates, lack of an effective therapeutic delivery system, low cell proliferation, and cell migration. In addition, long-term use of antibiotics in chronic wound management can result in side effects and antimicrobial resistance. Novel treatments with antibacterial pharmaceuticals thus vitally need to be developed. Recently, graphene and graphene family members have emerged as shining stars among biomaterials for wound-healing applications due to their excellent bioactive properties, which can overcome limitations of current wound dressings and fulfill wound-healing requirements. Herein, we developed a feasible approach to impregnate graphene oxide (GO) into genipin-crosslinked gelatin (3GO) hydrogels to enzymatically control GO release. The developed hydrogels were characterized by chemical, physical, morphological, and cellular analyses. The results proved that the 3GO1 hydrogel is biocompatible and significantly enhanced the mechanical strength by encapsulating GO. Moreover, the rate of GO release depended on the crosslinking degree and environmental enzyme levels. Enzymatically released GO displayed uniform dispersity, retained its antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa through sharp edges and wrapping mechanisms, and promoted human fibroblast migration. This multifunctional hydrogel we developed with antibacterial efficacy is suitable for future application as wound dressings.

引用

页数：12