A Novel Bioassay for High-Throughput Screening Microorganisms with N-acyl Homoserine Lactone Degrading Activity

被引：8

作者：

Liu, Pengfu ^{[1
,2
]}

Gao, Yang ^{[1
]}

Huang, Wei ^{[1
]}

Shao, Zongze ^{[3
]}

Shi, Jiping ^{[2
]}

Liu, Ziduo ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[2] Chinese Acad Sci, Shanghai Adv Res Inst, Sustainable Technol Res Ctr, Shanghai 201203, Peoples R China

[3] State Ocean Adm, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 167卷 / 01期

关键词：

N-Acyl homoserine lactone; Quorum sensing; Quorum quenching; Bioassay; AHL-degrading bacterium; SIGNAL MOLECULES; ACYLHOMOSERINE LACTONASE; QUORUM; STRAIN; BACTERIA; FAMILY;

D O I：

10.1007/s12010-012-9653-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel biosensor strain ( ALM403) that responded to -acyl homoserine lactone (AHL) was constructed using a cassette as a regulatory sequence and beta-mannanase as a reporter gene. Dinitrosalicylic acid method was used to detect the response of the sensor strain to -acyl homoserine lactone. By investigating the response to a range of concentrations of beta-oxooctanoyl--homoserine lactone (OOHL), it was demonstrated that the expression of mannanase in ALM403 could be greatly enhanced by OOHL and resulted in an assayable phenotype. A high-throughput screening approach was developed to isolate AHL-degrading microorganisms, and a marine sp. S66-4 showing a marked AHL-degrading ability was successfully isolated. In conclusion, the bioassay system provided a simple and efficient approach to isolate AHL-degrading bacteria.

引用

页码：73 / 80

页数：8

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