Preparation and characterization of a new p-allylealix[4]arene-bonded silica gel stationary phase for high-performance liquid chromatography

For the first time calix[4] arene tetraamide bonded stationary phase was prepared by modifying silica gel with 3-mercaptopropyltriethoxysilane and coupling with p-allylealix[4]arene tetraamide. The separation of polycyclic aromatic hydrocarbons, positional isomers of nitroaniline, benzoic esters and plithalic esters on the new calix[ 4 ] arenebonded stationary phase was achieved with acetonitrile-water or methanol-water as mobile phase. A comparison with conventional reversed-phase C-18 columns shows a predominant reversed-phase character and obvious priority for separation of positional isomers. The possible separation mechanism has been investigated.