Reproducible Matrix Deposition for MALDI MSI Based on Open-Source Software and Hardware

被引：19

作者：

Stoeckli, Markus ^{[1
]}

Staab, Dieter ^{[1
]}

机构：

[1] Novartis Inst BioMed Res, CH-4056 Basel, Switzerland

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2015年 / 26卷 / 06期

关键词：

MALDI MSI; Imaging; Matrix; Spray; iMatrixSpray; Open-source; IMAGING MASS-SPECTROMETRY; SAMPLE PREPARATION; RESOLUTION; PROTEINS; TISSUE;

D O I：

10.1007/s13361-015-1099-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The new open-source software and hardware matrix deposition device named iMatrixSpray was optimized and specified for homogeneity, reproducibility, and sensitivity in MS imaging experiments. The results confirm the design claims, with the device delivering uniform coatings with a constant quality from experiment to experiment. The robustness in combination with the open design allows developing and sharing of matrix deposition and sample preparation protocols between labs. This tool therefore enables researchers to enter the field of MALDI MSI without previous experience in matrix coating.

引用

页码：911 / 914

页数：4

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