Effect of tensile force on the expression of IGF-I and IGF-I receptor in the organ-cultured rat cranial suture

Insulin-like growth factors (IGFs) play an important role in the regulation of bone metabolism. In this study, we investigated changes in the expression of IGF-1 and IGF-1 receptor and cell proliferation when a continuous tensile force was applied to the cranial suture of cultured rat calvaria. The parietal bones with the midsagittal. suture were removed from mate Wistar rats (19-days old), cultured for 24 h, and divided into two groups. In the experimental. group, tensile force (3 x 10(-3) N) was applied by helical springs to the midsagittal. suture, whereas helical springs with no tension (0 N) were set in the control group. The tensile force significantly increased the expression of both IGF-1 mRNA and protein (P < 0.05). By using in situ hybridisation, we also confirmed that IGF-1 and IGF-1 receptor mRNAs were localized in osteoblast-like and fibroblastic cells subjected to the tensile force. Also, this force stimulated the proliferation of osteoblast-like and fibroblastic cells in calvaria, without affecting their alkaline phosphatase activity. These results indicate that a tensile force applied to a cranial suture can cause an increase in the production of IGF-1 and IGF-1 receptors in osteoblast-like and fibroblastic cells, and this increase in IGF-1 may cause the proliferation of the cells in an autocrine or paracrine manner. (c) 2004 Elsevier Ltd. All rights reserved.