Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation

被引：33
|
作者：
Kakizaki, Ikuko [1 ,3 ]
Itano, Naoki [4 ,5 ]
Kimata, Koji [6 ]
Hanada, Katsumi [2 ]
Kon, Atsushi [1 ]
Yamaguchi, Masanori [1 ]
Takahashi, Teruno [1 ]
Takagaki, Keiichi [1 ]
机构：
[1] Hirosaki Univ, Sch Med, Dept Biochem, Hirosaki, Aomori 0368562, Japan
[2] Hirosaki Univ, Sch Med, Dept Dermatol, Hirosaki, Aomori 0368562, Japan
[3] Hirosaki Univ, Grad Sch Med, Dept Glycotechnol, Ctr Adv Med Res, Hirosaki, Aomori 0368562, Japan
[4] Shinshu Univ, Dept Mol Oncol, Div Mol & Cellular Biol, Inst Aging & Adapt, Matsumoto, Nagano 3908621, Japan
[5] Aichi Med Univ, CREST, Japan Sci & Technol Agcy, Aichi 4801195, Japan
[6] Aichi Med Univ, Sch Med, Inst Mol Sci Med, Aichi 4801195, Japan
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2008年 / 471卷 / 01期
关键词：
hyaluronan; hyalronan synthase; UVB; keratinocytes; cytokines;
D O I：
10.1016/j.abb.2007.12.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and both were up-regulated after a single irradiation with moderate UVB but hyaluronidases was unchanged. Increased accumulation of hyaluronan in the culture medium mirrored the UVB-induced increase in the mRNA levels of HAS3 and HAS2. Unexpectedly, hyaluronan derived from UVB-irradiated and non-irradiated cells had identical size distribution. Increased expression of KGF and IL-1 beta was detected just prior to the increase of HAS3 and HAS2 mRNAs after UVB irradiation. Antibody-neutralization study revealed that KGF and/or IL-1 beta were at least involved in the up-regulation of HAS3 and HAS2 expressions. UVB-irradiated cells may enhance hyaluronan production to maintain homeostasis through up-regulation of HAS3 and HAS2 genes via cytokine response mechanism. (c) 2007 Elsevier Inc. All rights reserved.
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收藏
页码：85 / 93
页数：9
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