Electrical detection of TATA binding protein at DNA-modified microelectrodes

被引：73
|
作者：
Gorodetsky, Alon A. [1 ]
Ebrahim, Ali [1 ]
Barton, Jacqueline K. [1 ]
机构：
[1] CALTECH, Dept Chem & Chem Engn, Pasadena, CA 91125 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 10期
关键词：
D O I：
10.1021/ja7106756
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple method for the electrochemical detection of TATA-binding protein is demonstrated at DNA-modified microelectrodes. The assay is general and based on the interruption of DNA-mediated charge transport to Nile Blue, a redox-active probe covalently attached to the DNA base pair stack. Nanomolar quantities of TATA binding protein can be detected on the microelectrodes even in the presence of micromolar amounts of bovine serum albumin, Endonuclease III, or Bam HI methyltransferase. The scheme outlined provides a basis for the sensitive electrical detection of numerous proteins on a single DNA chip.
引用
收藏
页码：2924 / 2925
页数：2
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