Comparison of the chromatographic properties of IgG1 and IgG2 antibody subclasses

被引：1

作者：

Ghose, Sanchayita ^{[1
]}

Ladwig, Justin ^{[1
]}

Allen, Martin ^{[2
]}

机构：

[1] Amgen Inc, Purificat Proc Dev, Seattle, WA 98119 USA

[2] Amgen Inc, Cell Sci & Technol, Seattle, WA 98119 USA

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2010年 / 57卷

关键词：

antibody subclass; cation-exchange chromatography (CEX); chromatographic properties; IgG1; IgG2; A AFFINITY-CHROMATOGRAPHY; MONOCLONAL-ANTIBODIES; PROTEIN; THERAPEUTICS; PURIFICATION; SELECTION; ISOTYPE; DESIGN;

D O I：

10.1042/BA20100205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All therapeutic antibodies that are currently approved or under development are IgGs, with IgG1 and IgG2 being the most prevalent subclasses. The present study investigates the effect of antibody subclass on its chromatographic properties. To this end, two sets of antibodies with identical variable domains and differing subclasses (IgG1 and IgG2) were compared by using purification methods that are most commonly used in industrial antibody manufacturing processes. Each antibody was examined using Protein A chromatography, cation-exchange chromatography, hydrophobic interaction chromatography and salt-induced precipitation studies. The results of these experiments show that the variable region of an antibody has a greater influence on its chromatographic behaviour than antibody subclass.

引用

页码：111 / 116

页数：6

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