Effects of Shenmai injection on neuronal apoptosis related gene expression in rat brain after intracerebral hemorrhage

With Sprapue-Dewley rat models with intracerebral hemorrhage(ICH), the effects of Shenmai(SM) injection on the pathologic changes of the neuronal structure in hippocampal gyrus CA(1) areas, expression of Bcl-2 mRNA, Bax mRNA, c-myc mRNA, PDGF A mRNA and deletion in the areas around the hematoma are studied, The results show: 1) 7 d after ICH in the rats, we examined the brain tissues of the rats and found that SM injection had effects on antagonizing the pyramidal cells apoptosis in the hippocampal CA, areas and alleviating the pathologic signs of the neuronal system in rat models with ICH. It is suggested that SM injection could antagonize the neuronal apoptosis in rat models with ICH. 2) The levels of Bax mRNA and Bcl-2 mRNA increased significantly in rats 12 to 48 h after ICH with a peak at 24th h after ICH. SM injection could increase the levels of Bcl-2 mRNA and decrease the levels of Bax mRNA. It is suggested that SM injection could antagonize the neuronal apoptosis by regulating the expression of Bcl-2 mRNA and Bax MRNA. 3) The levels of c-myc mRNA and PDGF-A mRNA reached the peak 12 h after ICH. SM injection had no significant effect on the expression of c myc mRNA at initial phase, but decreased the levels of PDGF-A mRNA and prolonged the time of expression. It is suggested that SM injection could antagonize the delayed injury of the neuronal cells by regulating the expression of PDGF.