Determination of organic acids in fructus mume by high performance liquid chromatography

被引：0

作者：

Guo, Y

Xiao, ZP

Wang, H

Huang, TB ^{[1
]}

机构：

[1] Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2004年 / 32卷 / 12期

关键词：

reversed-phase high performance liquid chromatography; fructus mume; organic acid;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high performance liquid chromatographic method was developed for determining malic acid, lactic acid, acetic acid, citric acid and succinic acid in Fructus mume on a Kromasil C-18 column with 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.7) as mobile phase and ultraviolet detector at 215 nm. The column temperature was 17 degreesC and the flow rate was 0.5 mL/min. The results of the determination of organic acids in four kinds of Fructus mume were reported. The average recoveries of the method ranged from 98% to 105% with the relative standard deviation of 2.3% similar to 7.8% and detection limit 10 similar to 75 ng (S/N = 3).

引用

页码：1624 / 1626

页数：3