Trace eyeblink conditioning requires the hippocampus but not autophosphorylation of αCaMKII in mice

Little is known about signaling mechanisms underlying temporal associative learning. Here, we show that mice with a targeted point mutation that prevents autophosphorylation of alpha CaMKII (alpha CaMKIIT286A) learn trace eyeblink conditioning normally. This forms a sharp contrast to the severely impaired spatial learning in the water maze and contextual fear conditioning observed in alpha CaMKIIT286A mutants. Importantly, hippocampal lesions impaired trace eyeblink conditioning in alpha CaMKIIT286A mice, suggesting a potential role of hippocampal alpha CaMKII-independent mechanisms. These results indicate that hippocampal signaling mechanisms that underlie temporal associative learning as assessed by trace eyeblink conditioning may differ from those of spatial and contextual learning.