New strategy of cloning of differentially expressed genes.

被引：0

作者：

Cui, DX ^{[1
]}

Yan, XJ ^{[1
]}

Wang, F ^{[1
]}

Su, CZ ^{[1
]}

机构：

[1] Fourth Mil Med Univ PLA, Inst Genet Diag, Xian 710032, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2000年 / 27卷 / 04期

关键词：

gene expression; cloning; strategy;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There are two kinds of changes of gene expression, that is, novel gene expression and differential gene expression in quantity. Cloning technique of differentially expressed gene in quantity mainly is mRNA differential display, which is presently one of the most effective methods. However, mRNA differential display possesses higher unreal positive rate, in order to overcame its shortcoming, some novel strategies and methods were advocated, such as differential subtraction display, subtraction based on LD-PCR, LD-PCR based on subtraction, those techniques have dominant advantages to mRNA differential display.

引用

页码：362 / 364

页数：3

共 11 条

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