Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein

被引：37
|
作者：
Spencer, Kelly-Anne [1 ]
Dee, Michael [2 ]
Britton, Paul [2 ]
Hiscox, Julian A. [1 ,3 ]
机构：
[1] Univ Leeds, Fac Biol Sci, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Inst Anim Hlth, Div Microbiol, Newbury RG20 7NN, Berks, England
[3] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
来源：
VIROLOGY | 2008年 / 370卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
coronavirus; RNA binding; nucleocapsid protein; IBV; surface plasmon resonance; phosphorylation;
D O I：
10.1016/j.virol.2007.08.016
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster was involved in the recognition of viral RNA from non-viral RNA. The IBV N protein has been found to be essential for the successful recovery of IBV using reverse genetics systems. Rescue experiments indicated that phosphorylated N protein recovered infectious IBV more efficiently when compared to modified N proteins either partially or non-phosphorylated. Our data indicate that the phosphorylated form of the IBV N protein plays a role in virus biology. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：373 / 381
页数：9
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