Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress

被引:12
|
作者
Zhang, Liang [1 ]
Gong, Xianzhe [2 ]
Chen, Zhiyi [2 ,3 ]
Zhou, Yan [1 ,4 ]
机构
[1] Nanyang Technol Univ, Nanyang Environm & Water Res Inst, Adv Environm Biotechnol Ctr, Singapore 637141, Singapore
[2] Shandong Univ, Inst Marine Sci & Technol, Qingdao 266237, Shandong, Peoples R China
[3] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
[4] Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore 639798, Singapore
基金
中国国家自然科学基金;
关键词
Anaerobicdigestion; Methanogenesis; Syntrophicoxidation; Propionateaccumulation; Ammoniastress; SLUDGE; PERFORMANCE; TOOL; INSIGHTS; WASTE; NOV;
D O I
10.1016/j.biortech.2022.127574
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The ecological roles of microbial communities and how they interact with each other in thermal hydrolysis process (THP) assisted thermophilic anaerobic digestion (THP-AD) reactors remain largely unknown, especially under propionate stress. Two thermophilic THP-AD reactors had methane yield of 240-248 mL/g VSadded, but accumulated approximately 2000 mg/L propionate. Genome-centric metagenomics analysis showed that 68 metagenome-assembled genomes (MAGs) were recovered, 32 MAGs of which were substantially enriched. Fir-micutes spp. dominated the enriched microbial community, including hydrolytic/fermentative bacteria and syntrophs. Methanogenic activities were mainly mediated by Methanosarcina sp. and Methanothermobacter spp. In addition to hydrogenotrophic methanogens, Thermodesulfovibrio sp. could also be a vital H-2 scavenger, contributing to maintaining low H-2 partial pressure in the bioreactors. The remarkable accumulation of propionate could be likely attributed to the weak syntrophic propionate-oxidizing activity or its absence. These findings advanced our knowledge about the mutualistic symbiosis of carbon metabolism in thermophilic THP-AD reactors.
引用
收藏
页数:10
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