Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry

被引：3

作者：

Wang, Longwen ^{[1
]}

Ma, Jimei ^{[1
]}

Cheng, Xin ^{[1
]}

Li, Zilong ^{[1
]}

Sun, Linhao ^{[1
]}

Zeng, Zhen ^{[1
]}

Jiang, Hong ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Sci, Dept Chem, Wuhan 430070, Hubei, Peoples R China

来源：

CHINESE JOURNAL OF ORGANIC CHEMISTRY | 2018年 / 38卷 / 10期

基金：

中国国家自然科学基金;

关键词：

beta-glucosidase activity; determination; 2-O-(beta-glucopyranosyl)ascorbic acid; click chemistry; PROBE;

D O I：

10.6023/cjoc201803011

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A novel method to quantify beta-glucosidase activity was developed by coupling the cleavage of 2-O-(beta-glucopyranosyl)ascorbic acid with the reduction of Cu (II). The in situ generated Cu(I) catalyzed the cycloaddition between weakly fluorescent coumarin and benzyl azide to yield a highly fluorescent triazole product. The fluorescence intensity was dependently enhanced on the increase of the activity of beta-glucosidase and a linear relationship was found between 1 similar to 40 U/L. This cascade allows detection of beta-glucosidase with a limit of detection of 0.456 U/L.

引用

页码：2775 / 2779

页数：5

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