Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism

被引:11
|
作者
Grande, Giuseppe [1 ,2 ]
Vincenzoni, Federica [3 ,4 ]
Mancini, Francesca [1 ]
Barrachina, Ferran [5 ,6 ]
Giampietro, Antonella [2 ]
Castagnola, Massimo [7 ]
Urbani, Andrea [3 ,4 ]
Oliva, Rafael [5 ,6 ]
Milardi, Domenico [1 ,2 ]
Pontecorvi, Alfredo [1 ,2 ]
机构
[1] Int Sci Inst Paul VI, I-100168 Rome, Italy
[2] Fdn Policlin Univ Agostino Gemelli IRCCS, Div Endocrinol, I-00168 Rome, Italy
[3] Univ Cattolica Sacro Cuore, Ist Biochim & Biochim Clin, I-100168 Rome, Italy
[4] Fdn Policlin Univ Agostino Gemelli IRCCS, Dipartimento Sci Lab & Infettivol, I-00168 Rome, Italy
[5] Univ Barcelona, Mol Biol Reprod & Dev Res Grp, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Dept Biomed Sci,Fac Med & Hlth Sci, E-08036 Barcelona, Spain
[6] Hosp Clin Barcelona, Biochem & Mol Genet Serv, Barcelona 08036, Spain
[7] IRCCS Fdn Santa Lucia, Lab Prote & Metabol, I-100168 Rome, Italy
关键词
seminal plasma; proteomics; testosterone; testosterone deficiency; hypogonadism; accessory glands; PROSTATIC SECRETORY PROTEIN; C INHIBITOR; BETA-MICROSEMINOPROTEIN; ANDROGEN DEFICIENCY; COMPLEX-FORMATION; IN-VITRO; TISSUE; LOCALIZATION; PURIFICATION; FERTILITY;
D O I
10.3390/jcm8122128
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.
引用
收藏
页数:13
相关论文
共 50 条
  • [1] In-depth quantitative proteome analysis of seminal plasma from men with oligoasthenozoospermia and normozoospermia
    Liu, Xin
    Wang, Wenting
    Zhu, Peng
    Wang, Jiahui
    Wang, Yanwei
    Wang, Xuebo
    Liu, Juan
    Li, Ning
    Wang, Xiong
    Lin, Chunhua
    Liu, Fujun
    [J]. REPRODUCTIVE BIOMEDICINE ONLINE, 2018, 37 (04) : 467 - 479
  • [2] Analysis of bull (Bos taurus) seminal vesicle fluid proteome in relation to seminal plasma proteome
    Westfalewicz, B.
    Dietrich, M. A.
    Mostek, A.
    Partyka, A.
    Bielas, W.
    Nizanski, W.
    Ciereszko, A.
    [J]. JOURNAL OF DAIRY SCIENCE, 2017, 100 (03) : 2282 - 2298
  • [3] Bioinformatics approaches for improving seminal plasma proteome analysis
    Noor, Zainab
    Ranganathan, Shoba
    [J]. THERIOGENOLOGY, 2019, 137 : 43 - 49
  • [4] Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance
    Mills, Kayla M.
    Aryal, Uma K.
    Sobreira, Tiago
    Minton, Amanda M.
    Casey, Theresa
    Stewart, Kara R.
    [J]. THERIOGENOLOGY, 2020, 157 : 130 - 139
  • [5] Proteome analysis of Nelore bull (Bos taurus indicus) seminal plasma
    Assumpçao, TI
    Fontes, W
    Sousa, MV
    Ricart, CAO
    [J]. PROTEIN AND PEPTIDE LETTERS, 2005, 12 (08): : 813 - 817
  • [6] Characterization of carp seminal plasma proteome in relation to blood plasma
    Dietrich, Mariola A.
    Arnold, Georg J.
    Nynca, Joanna
    Froehlich, Thomas
    Otte, Kathrin
    Ciereszko, Andrzej
    [J]. JOURNAL OF PROTEOMICS, 2014, 98 : 218 - 232
  • [7] Comparative analysis of carp seminal plasma proteome from different freezability semen
    Dietrich, M.
    Irnazarow, I.
    Jurecka, P.
    Dietrich, G.
    Karol, H.
    Liszewska, E.
    Ciereszko, A.
    [J]. REPRODUCTION IN DOMESTIC ANIMALS, 2016, 51 : 61 - 61
  • [8] SHOTGUN PROTEOME ANALYSIS OF SEMINAL PLASMA PROTEINS DIFFERENTIATES BOARS BY REPRODUCTIVE PERFORMANCE
    Stewart, Kara R.
    Casey, Theresa
    Mills, Kayla
    Aryal, Uma K. Uma
    Minton, Amanda M.
    [J]. THERIOGENOLOGY, 2019, 137 : 132 - 132
  • [9] Seminal plasma metabolomics signatures of normosmic congenital hypogonadotropic hypogonadism
    Li, Xiaogang
    Guo, Ye
    Wang, Xi
    Li, Haolong
    Mao, Jiangfeng
    Yan, Songxin
    Luo, Guoju
    Wang, Renzhi
    Wu, Xueyan
    Li, Yongzhe
    [J]. HELIYON, 2023, 9 (04)
  • [10] Quantitative analysis of the sHLA-G protein in seminal plasma
    Schallmoser, A.
    Saenger, N.
    [J]. HUMAN REPRODUCTION, 2020, 35 : 145 - 145