A highly selective quinolizinium-based fluorescent probe for cysteine detection

被引：11
|
作者：
Wa-Yi, O. [1 ,2 ]
Chan, Wing-Cheung [2 ]
Xu, Caifeng [1 ]
Deng, Jie-Ren [2 ]
Ko, Ben Chi-Bun [2 ]
Wong, Man-Kin [1 ,2 ]
机构：
[1] Hong Kong Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China
[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chem Biol & Drug Discovery, Hung Hom, Hong Kong, Peoples R China
来源：
RSC ADVANCES | 2021年 / 11卷 / 53期
关键词：
TURN-ON; LIQUID-CHROMATOGRAPHY; TOTAL HOMOCYSTEINE; FACILE PREPARATION; LIVING CELLS; HUMAN PLASMA; GLUTATHIONE; THIOLS; ELECTROPHORESIS; CHEMODOSIMETER;
D O I：
10.1039/d1ra06104f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 mu M (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).
引用
收藏
页码：33294 / 33299
页数：6
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