Reductive dehalogenation of brominated phenolic compounds by microorganisms associated with the marine sponge Aplysina aerophoba

被引:96
|
作者
Ahn, YB
Rhee, SK
Fennell, DE
Kerkhof, LJ
Hentschel, U
Häggblom, MM [1 ]
机构
[1] Rutgers State Univ, Dept Biochem, New Brunswick, NJ 08903 USA
[2] Rutgers State Univ, Microbiol & Biotechnol Ctr Agr & Environm, Inst Marine & Coastal Sci, New Brunswick, NJ 08903 USA
[3] Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08903 USA
[4] Univ Wurzburg, Inst Infekt Biol, D-97070 Wurzburg, Germany
关键词
D O I
10.1128/AEM.69.7.4159-4166.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Marine sponges are natural sources of brominated organic compounds, including bromoindoles, bromophenols, and bromopyrroles, that may comprise up to 12% of the sponge dry weight. Aplysina aerophoba sponges harbor large numbers of bacteria that can amount to 40% of the biomass of the animal. We postulated that there might be mechanisms for microbially mediated degradation of these halogenated chemicals within the sponges. The capability of anaerobic microorganisms associated with the marine sponge to transform haloaromatic compounds was tested under different electron-accepting conditions (i.e., denitrifying, sulfidogenic, and methanogenic). We observed dehalogenation activity of sponge-associated microorganisms with various haloaromatics. 2-Bromo-, 3-bromo-, 4-bromo-, 2,6-dibromo-, and 2,4,6-tribromophenol, and 3,5-dibromo-4-hydroxybenzoate were reductively debrominated under methanogenic and sulfidogenic conditions with no activity observed in the presence of nitrate. Monochlorinated phenols were not transformed over a period of 1 year. Debromination of 2,4,6-tribromophenol, and 2,6-dibromophenol to 2-bromophenol was more rapid than the debromination of the monobrominated phenols. Ampicillin and chloramphenicol inhibited activity, suggesting that dehalogenation was mediated by bacteria. Characterization of the debrominating methanogenic consortia by using terminal restriction fragment length polymorphism (TRFLP) and denaturing gradient gel electrophoresis analysis indicated that different 16S ribosomal DNA (rDNA) phylotypes were enriched on the different halogenated substrates. Sponge-associated microorganisms enriched on organobromine compounds had distinct 16S rDNA TRFLP patterns and were most closely related to the delta subgroup of the proteobacteria. The presence of homologous reductive dehalogenase gene motifs in the sponge-associated microorganisms suggested that reductive dehalogenation might be coupled to dehalorespiration.
引用
收藏
页码:4159 / 4166
页数:8
相关论文
共 50 条
  • [1] Bacterial Uptake by the Marine Sponge Aplysina aerophoba
    Markus Wehrl
    Michael Steinert
    Ute Hentschel
    [J]. Microbial Ecology, 2007, 53 : 355 - 365
  • [2] Bacterial uptake by the marine sponge Aplysina aerophoba
    Wehrl, Markus
    Steinert, Michael
    Hentschel, Ute
    [J]. MICROBIAL ECOLOGY, 2007, 53 (02) : 355 - 365
  • [3] Wound Activation of Protoxins in Marine Sponge Aplysina aerophoba
    Rainer Ebel
    Marko Brenzinger
    Arno Kunze
    Hans J. Gross
    Peter Proksch
    [J]. Journal of Chemical Ecology, 1997, 23 : 1451 - 1462
  • [4] Wound activation of protoxins in marine sponge Aplysina aerophoba
    Ebel, R
    Brenzinger, M
    Kunze, A
    Gross, HJ
    Proksch, P
    [J]. JOURNAL OF CHEMICAL ECOLOGY, 1997, 23 (05) : 1451 - 1462
  • [5] Distribution of brominated compounds within the sponge Aplysina aerophoba: coupling of X-ray microanalysis with cryofixation techniques
    Xavier Turon
    Mikel A. Becerro
    María J. Uriz
    [J]. Cell and Tissue Research, 2000, 301 : 311 - 322
  • [6] Distribution of brominated compounds within the sponge Aplysina aerophoba:: coupling of X-ray microanalysis with cryofixation techniques
    Turon, X
    Becerro, MA
    Uriz, MJ
    [J]. CELL AND TISSUE RESEARCH, 2000, 301 (02) : 311 - 322
  • [7] ANTIBIOTIC AND CYTOTOXIC ACTIVITY OF BROMINATED COMPOUNDS FROM THE MARINE SPONGE VERONGIA-AEROPHOBA
    TEEYAPANT, R
    WOERDENBAG, HJ
    KREIS, P
    HACKER, J
    WRAY, V
    WITTE, L
    PROKSCH, P
    [J]. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 1993, 48 (11-12): : 939 - 945
  • [8] BIOTRANSFORMATION OF BROMINATED COMPOUNDS IN THE MARINE SPONGE VERONGIA-AEROPHOBA - EVIDENCE FOR AN INDUCED CHEMICAL DEFENSE
    TEEYAPANT, R
    PROKSCH, P
    [J]. NATURWISSENSCHAFTEN, 1993, 80 (08) : 369 - 370
  • [9] Temporal variation of the microbial community associated with the mediterranean sponge Aplysina aerophoba
    Friedrich, AB
    Fischer, I
    Proksch, P
    Hacker, J
    Hentschel, U
    [J]. FEMS MICROBIOLOGY ECOLOGY, 2001, 38 (2-3) : 105 - 113
  • [10] Complete reductive dehalogenation of brominated biphenyls by anaerobic microorganisms in sediment
    Bedard, DL
    Van Dort, HM
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1998, 64 (03) : 940 - 947