Double-μPeriscope, a tool for multilayer optical recordings, optogenetic stimulations or both

被引:0
|
作者
Suzuki, Mototaka [1 ,3 ]
Aru, Jaan [1 ,2 ]
Larkum, Matthew E. [1 ]
机构
[1] Humboldt Univ, Inst Biol, Berlin, Germany
[2] Univ Tartu, Inst Comp Sci, Tartu, Estonia
[3] Univ Amsterdam, Swammerdam Inst Life Sci, Amsterdam, Netherlands
来源
ELIFE | 2021年 / 10卷
基金
欧洲研究理事会;
关键词
neurons; cortex; thalamus; optogenetics; calcium imaging; Mouse; CELLULAR RESOLUTION; CRE-RECOMBINASE; POPULATIONS; LAYERS; MICE;
D O I
10.7554/eLife.72894; 10.7554/eLife.72894.sa0; 10.7554/eLife.72894.sa1; 10.7554/eLife.72894.sa2
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intelligent behavior and cognitive functions in mammals depend on cortical microcircuits made up of a variety of excitatory and inhibitory cells that form a forest-like complex across six layers. Mechanistic understanding of cortical microcircuits requires both manipulation and monitoring of multiple layers and interactions between them. However, existing techniques are limited as to simultaneous monitoring and stimulation at different depths without damaging a large volume of cortical tissue. Here, we present a relatively simple and versatile method for delivering light to any two cortical layers simultaneously. The method uses a tiny optical probe consisting of two microprisms mounted on a single shaft. We demonstrate the versatility of the probe in three sets of experiments: first, two distinct cortical layers were optogenetically and independently manipulated; second, one layer was stimulated while the activity of another layer was monitored; third, the activity of thalamic axons distributed in two distinct cortical layers was simultaneously monitored in awake mice. Its simple-design, versatility, small-size, and low-cost allow the probe to be applied widely to address important biological questions.
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页数:10
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