BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

被引：7
|
作者：
Verma, Sonam [1 ]
Kang, Amandeep Kaur [1 ,3 ]
Pal, Rahul [2 ]
Gupta, Satish Kumar [1 ]
机构：
[1] Natl Inst Immunol, Reprod Cell Biol Lab, Aruna Asaf Ali Marg, New Delhi 110067, India
[2] Natl Inst Immunol, Immunoendocrinol Lab, New Delhi, India
[3] Univ Delhi, Dr BR Ambedkar Ctr Biomed Res, Dept Biomed Sci, New Delhi 110007, India
来源：
CELL ADHESION & MIGRATION | 2020年 / 14卷 / 01期
关键词：
IFN-gamma; BST2; trophoblast invasion; E-cadherin; STAT1; AKT; ACTIVATED PROTEIN-KINASE; PHOSPHATIDYLINOSITOL; 3-KINASE; IFN-GAMMA; BREAST-CANCER; MESENCHYMAL TRANSITION; TROPHOBLAST INVASION; GROWTH; MIGRATION; TETHERIN; MOTILITY;
D O I：
10.1080/19336918.2019.1710024
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The mechanism by which interferon-gamma (IFN-gamma) downregulates trophoblast invasion needs further investigation. Treatment of HTR-8/SVneo cells with IFN-gamma led to a decrease in their invasion concomitant with an increased expression of BST2. Silencing of BST2 by siRNA showed a significant increase in their invasion and spreading after treatment with IFN-gamma as well as downregulated expression of E-cadherin. Further, STAT1 silencing inhibited the IFN-gamma-dependent increase in the expression of BST2 and E-cadherin. Treatment of HTR-8/SVneo cells with IFN-gamma led to the activation of AKT, and its inhibition with PI3K inhibitor abrogated IFN-gamma-mediated decrease in invasion/spreading and downregulated BST2 and E-cadherin expression. Collectively, IFN-gamma decreases the invasion of HTR-8/SVneo cells by STAT1 and AKT activation via increased expression of BST2 and E-cadherin.
引用
收藏
页码：24 / 41
页数：18
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