Non-linear 3D microscopy of ex-vivo corneas

被引：2
|
作者：
Bueno, Juan M. [1 ]
Gualda, Emilio J. [1 ]
Artal, Pablo [1 ]
机构：
[1] Univ Murcia, Lab Opt, E-30100 Murcia, Spain
来源：
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES X | 2010年 / 7569卷
关键词：
Multiphoton microscopy; second harmonic generation; two-photon fluorescence; cornea; 2ND-HARMONIC GENERATION; FLUORESCENCE;
D O I：
10.1117/12.840720
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A multiphoton microscope has been developed to investigate the sources of nonlinear fluorescence (TPEF) and second harmonic generation (SHG) in non-stained samples of ex-vivo corneas. Stacks of images from different depths are recorded to reconstruct high-resolution 3D (volume) images of the cornea. The corneal epithelium and endothelium provide significant TPEF signal, while the only source of SHG is the stroma. Within the stroma, the keratocytes can also be visualized. Volumetric 3D images of the cornea combining TPEF and SHG signals are useful to characterize the organization of the corneal collagen and to describe the distribution of keratocytes. These images will help to better understand how different pathologies modify the corneal structure and to control the changes produced by surgical or healing processes.
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页数：4
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