Influence of a glass-water interface on the on-axis trapping of micrometer-sized spherical objects by optical tweezers

被引：47

作者：

Fällman, E ^{[1
]}

Axner, O ^{[1
]}

机构：

[1] Umea Univ, Dept Phys, SE-90187 Umea, Sweden

来源：

APPLIED OPTICS | 2003年 / 42卷 / 19期

关键词：

D O I：

10.1364/AO.42.003915

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A systematic study of the influence of a glass-water interface on the on-axis trapping of micrometer-sized spherical objects by optical tweezers is presented. The ways in which the escape force and the trapping position, as well as the stiffness of the trap, depend on the focusing depth, the numerical aperture, and the degree of overfilling of the objective entrance pupil are investigated. It is concluded, among other things, that objectives with the highest numerical aperture and the use of large degrees of overfilling do not always provide the optimum trapping conditions at finite depths. (C) 2003 Optical Society of America.

引用

页码：3915 / 3926

页数：12