Conjugated linoleic acid suppresses the migratory and inflammatory phenotype of the monocyte/macrophage cell

Objective: We have previously shown that conjugated linoleic acid (CLA) regresses pre-established murine atherosclerosis. Although the exact underlying mechanisms are unclear, accumulation of macrophages and expression of inflammatory markers were reduced in atherosclerotic plaques of CLA-fed mice, implicating the monocyte/macrophage as a target through which CLA may mediate anti-atherosclerotic effects. CLA mediates its effect at least in part via activation of the nuclear receptor, peroxisome proliferator activator receptor-gamma (PPAR gamma). In this study we investigate if CLA mediates anti-atherogenic effects via modulation of monocyte/macrophage function and provide evidence for an additional PPAR gamma-independent mechanism for CLA. Methods and results: Migration of the human monocyte cell line THP-1, and primary blood monocytes (HPBMCs) was assessed using transwell migration assays. Monocyte chemoattractant protein-1 (MCP1) mediates chemotaxis via interaction with the chemokine (C-C motif)-2 receptor (CCR-2), which is expressed on the monocyte cell surface, and is negatively regulated by PPAR gamma agonists. Incubation of THP-1 monocytes with CLA-isomers and a PPAR gamma agonist inhibited MCP-1-induced monocyte migration. Prior to monocyte recruitment, activated platelets accumulate and release the contents of their secretory granules ("platelet-releasate"). Here we demonstrate that platelet-releasateis a monocyte chemoattractant, and CLA, but not the PPAR gamma agonist, inhibits platelet-releasate-induced migration of THP-1 and HPBMC monocytes. CLA-treatment also suppressed the inflammatory macrophage phenotype, demonstrated by decreased induction of monocyte migration by CLA-t reated macrophage-conditioned-media, as well as by decreased cyclooxygenase ( COX)-2 and cytosolic phospholipase-A(2) (cPLA(2)) expression and MCP-1, prostaglandin E-2 (PGE(2)) and matrix metalloprotease (MMP)-9 generation. Conclusions: CLA-isomers inhibit monocyte migration and reduce the inflammatory output of the macrophage. These mechanisms may contribute to the potent anti-atherosclerotic effects of CLA in vivo. (C) 2010 Elsevier Ireland Ltd. All rights reserved.