High-resolution separation of tubulin monomers on polyacrylamide minigels

被引：8
|
作者：
Banerjee, Abhijit [1 ]
Bovenzi, Frank A. [1 ]
Bane, Susan L. [1 ]
机构：
[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2010年 / 402卷 / 02期
关键词：
BETA-TUBULIN; ALPHA-TUBULIN; GEL-ELECTROPHORESIS; MULTIPLE FORMS; BRAIN; MICROTUBULES; SUBUNITS; YEAST;
D O I：
10.1016/j.ab.2010.03.035
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-resolution separation of alpha- and beta-tubulin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on minigels can be performed rapidly using simple modifications of the standard Laemmli procedure. Separation of the subunits can be observed even in high-protein loads (up to 40 mu g of protein). (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：194 / 196
页数：3
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