Enzymatic synthesis of non-digestible oligosaccharide catalyzed by dextransucrase and dextranase from maltose acceptor reaction

被引：18
|
作者：
Huang, Shuang-Xia [2 ]
Hou, Dian-Zhi [3 ]
Qi, Peng-Xiang [1 ]
Wang, Qing [1 ]
Chen, Hua-Lei [2 ]
Ci, Lu-Yu [1 ]
Chen, Shan [1 ]
机构：
[1] Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
[2] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
[3] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 523卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Maltose acceptor; Dextransucrase; Dextranase; Isomalto-oligosaccharides; Dextran; Molecular weight; FAECALIS ESAWY DEXTRANSUCRASE; ISOMALTO-OLIGOSACCHARIDES; LACTOBACILLUS-REUTERI; SYNERGISTIC CATALYSIS; OLIGODEXTRANS; BIOSYNTHESIS; OPTIMIZATION;
D O I：
10.1016/j.bbrc.2019.12.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Non-digestible oligosaccharides have wide food industrial applications as dietary fibers and prebiotics. The aim of this study is to realize the effective biosynthesis of isomalto-oligosaccharides (IMOs) and reduce the production of by-product dextran. In the presence of acceptors improved the dextransucrase reaction shifting to oligosaccharides formation but a number of by-products dextran appeared. Maltose acceptor performed stronger inhibition behaviors in dextran synthesis than lactose and glucose acceptor due to its higher efficiencies. Acceptors had no influence on the structure of by-product dextran which mainly composed of alpha-(1,6)-glycosidic linkages and low alpha-(1,3)-glycosidic branch. In addition, the Mw and contents of IMOs and oligodextrans synthesized by dual-enzyme were hard to control. Addition of maltose acceptor in the dual-enzyme reaction, the adequate dextranase preferentially degraded dextran than the acceptor products to yield the IMOs. Results indicated that the combined use of the dual-enzyme and the maltose acceptor is a simple and effective method to promote the high-quality of functional IMOs. (C) 2020 Elsevier Inc. All rights reserved.
引用
收藏
页码：651 / 657
页数：7
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