Alteration of cross-linking selectivity with the 2′-OMe analogue of 2-amino-6-vinylpurine and evaluation of antisense effects

被引：24

作者：

Imoto, Shuhei ^{[1
]}

Hori, Tsuneaki ^{[1
]}

Hagihara, Shinya ^{[1
]}

Taniguchi, Yosuke ^{[2
]}

Sasaki, Shigeki ^{[2
,3
]}

Nagatsugi, Fumi ^{[1
,3
]}

机构：

[1] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Aoba Ku, Sendai, Miyagi 9808577, Japan

[2] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan

[3] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2010年 / 20卷 / 20期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

Oligonucleotide; Cross-linking; Antisense; DNA; Nucleic acids chemistry; MODIFIED OLIGONUCLEOTIDES; INHIBITION; DNA; ACTIVATION; EFFICIENT; MICRORNA; STRATEGY;

D O I：

10.1016/j.bmcl.2010.08.027

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2'-OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2'-OMe oligonucleotides bearing 2 achieve highly selective cross-linking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：6121 / 6124

页数：4

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