Cytotoxicity Test of Isolated Compound from Syzygium malaccense Stem Bark on Fibroblast BHK 21 Cells

Medicinal plants are one of the traditional medicines that are now often used by the community. One plant that can be used as a medicinal plant is guava (Syzygium malaccense). The plant is a Syzygium genus that grows a lot in Java. Several studies have shown that stem bark of the plant contain sapiens, alkaloids, lycopene, ascorbic acid, beta carotene, and the most dominated by tannins which are antibacterial and antifungal. The objective of research: to prove the cytotoxicity test of isolated dichloromethane extract of guava (Syzygium malaccense) stem barks with a concentration of 12.5%, 25%, 50% and 100% of baby hamster kidney fibroblasts cell culture (BHK 21). Methods and materials: laboratory experimental research with Post Test Only using 6 treatments. research in the form of fibroblast BHK 21 cell culture as many as 6 groups, namely given isolated dichloromethane extract of Syzygium malaccense stem bark 12.5%, isolated dichloromethane extract of Syzygium Malaccans stem bark 25%, isolated dichloromethane extract of Syzygium malaccense stem bark 50%, and isolated dichloromethane extract of Syzygium malaccense stem bark 100%, cell control and media control. The research stages include BHK cell split stages and treatment stages. The reading results using Elisa Reader. Percentage of living cells using the Freshney formula). The results of the study: isolated dichloromethane extract of Syzygium malaccense stem bark 12.5% concentration had 79%, isolated dichloromethane extract of Syzygium malaccense stem bark 25% concentration had 77% live cells, 50% concentration had 75% live cells, and 100% concentration had 72% live cells. Conclusion: isolated dichloromethane extract of Syzygium malaccense stem bark 12.5%, 25%, 50% and 100% concentration not toxic to BHK 21 fibroblast cells.