Lipases are soluble and active in glycerol carbonate as a novel biosolvent

被引：29
|
作者：
Ou, Guangnan [1 ]
He, Biyan [1 ]
Yuan, Youzhu [2 ,3 ]
机构：
[1] Jimei Univ, Sch Bioengn, Xiamen 361021, Peoples R China
[2] Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China
[3] Xiamen Univ, Natl Engn Lab Green Chem Prod Alcohols Ethers Est, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2011年 / 49卷 / 02期
关键词：
Biosolvent; Ionization; Dissociation; Lipase; Activity; ORGANIC-SOLVENTS; ENZYME CATALYSIS; PROTEIN; ELECTROPHORESIS; STABILITY;
D O I：
10.1016/j.enzmictec.2011.04.011
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pK(a) value of trichloroacetic acid and transesterification activities of Candida antarctica lipase B and Candida rugosa lipase in glycerol carbonate are similar to those in water, indicating that ionizing and dissociating powers are capable of satisfactorily predicting the biocompatibility of organic solvents for soluble enzymatic systems. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：167 / 170
页数：4
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