PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES OF DIFFERENT EXTRACTS OF SELAGINELLA DOEDERLEINII

Five fractions of ethanol extract, i.e. petroleum ether extraction (PEE), diethyl ether extraction (DEE), ethyl acetate extraction (EAE), methyl alcohol extraction (MAE) and water extraction (WE), were subjected to evaluate antitumor, antioxidant and antibacterial effects. Total phenols and biflavonoids content were followed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) radical scavenging assay, ferric reducing power assay and chelation of ferrous ions assay for evaluating antioxidant potency. Among five fractions, EAE had efficiently high amount of total biflavonoids and MAE had higher phenols content. At 150 mu g/ml of different fractions, EAE exhibited 85.77% DPPH radical scavenging activity, 90.39% ABTS radical scavenging activity, 82.51% chelation of ferrous ions effect and 1.227 reducing power. PEE showed more efficient zone of inhibition against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas. In anticancer test, EAE showed marked cytotoxicity against A549 cell strain, 7721 cell strain, Hela cell line and Eca-109 cell line with IC50 value of 52.66, 66.20, 37.53 and 62.09 mu g/ml, respectively than the other four fractions. Biflavonoids were found to play an important role for anticancer effect of S. doederleinii with R-2 values of 0.915, 0.834, 0.818, 0.718, respectively and their coefficient values were significantly higher than that of phenols. The extracts of S. doederleinii contain remedial potential and can be used as possible source for drug development by pharmaceutical industries.