Trichobradybaena, gen. nov. (Gastropoda, Stylommatophora, Bradybacnidae) with three species, characterized by the association of a simple penial caecum on the penis near the penis-epiphallus transition and two mucus glands inserted on the dart sac, is described from the region of Yangtze River, China. Shell morphology and genitalia of all three species are described and figured. A distribution map and keys both to Chinese bradybaenid genera and to Trichobradybaena species are also provided. The morphology of the gonad gland, which shows consistent interspecific difference in Trichobradybaena, gen. nov., is here used for bradybaenid classification for the first time. The three members of the genus are T chagangensis, sp. nov., T tuberculata, sp. nov., and T submissa (Deshayes, 1873) comb. nov.: the latter is designated type species. Trichobradybaena submissa is widespread in several mid-western provinces of China. The other two species are both narrowly distributed on the eastern slope of the Qinghai-Tibet Plateau and only known from the type material. All type specimens involved are deposited at the Museum of the Institute of Zoology, Chinese Academy of Sciences.