A Microfluidic Device for Rapid Screening of E-coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis

被引：15

作者：

Ngamsom, Bongkot ^{[1
]}

Truyts, Alma ^{[2
]}

Fourie, Louis ^{[2
]}

Kumar, Shavon ^{[2
]}

Tarn, Mark D. ^{[1
]}

Iles, Alexander ^{[1
]}

Moodley, Klariska ^{[2
]}

Land, Kevin J. ^{[2
]}

Pamme, Nicole ^{[1
]}

机构：

[1] Univ Hull, Sch Math & Phys Sci, Cottingham Rd, Kingston Upon Hull HU6 7RX, N Humberside, England

[2] CSIR, Meiring Naude Rd, ZA-0184 Pretoria, South Africa

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2017年 / 23卷 / 52期

关键词：

ATP assays; E; coli; immunoassays; luminescence; water chemistry; IN-SITU HYBRIDIZATION; SOLID-PHASE CYTOMETRY; DRINKING-WATER; SURFACE-TENSION; BACTERIA; SAMPLES; FLOW; AMPLIFICATION; ENUMERATION; CAPTURE;

D O I：

10.1002/chem.201703487

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a simple microfluidic system for rapid screening of Escherichia coli (E.coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E.coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(4)CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.

引用

页码：12754 / 12757

页数：4

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