Unanticipated changes in steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase in rat tibiae

被引：5

作者：

Maran, A ^{[1
]}

Hefferan, TE ^{[1
]}

Zhang, M ^{[1
]}

Turner, RT ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Dept Orthoped, Rochester, MN 55905 USA

来源：

CALCIFIED TISSUE INTERNATIONAL | 2004年 / 74卷 / 02期

关键词：

glyceraldehyde-3-phosphate dehydrogenase; (GAPDH); mechanical unloading; rat skeleton; control gene;

D O I：

10.1007/s00223-002-1098-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels are commonly used as an internal control to normalize gene expression data based on the belief that this gene is constitutively expressed. However, GAPDH mRNA levels increased by more than 2.5-fold in tibiae of hindlimb unloaded female rats compared to L32 mRNA levels. Similarly, GAPDH mRNA levels increased compared to 18S ribosomal RNA. Treatment with growth hormone and alcohol show no disparity in GAPDH mRNA levels whereas in some experiments, parathyroid hormone and 17beta-estradiol increased GAPDH mRNA levels. Taken together, these findings indicate that it is essential to demonstrate that GAPDH expression is not altered prior to using the gene as an internal control.

引用

页码：204 / 207

页数：4

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