Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

被引：17

作者：

Bergmann, S. M. ^{[1
]}

Wang, Q. ^{[2
]}

Zeng, W. ^{[2
]}

Li, Y. ^{[2
]}

Wang, Y. ^{[2
]}

Matras, M. ^{[3
]}

Reichert, M. ^{[3
]}

Fichtner, D. ^{[1
]}

Lenk, M. ^{[1
]}

Morin, T. ^{[4
]}

Olesen, N. J. ^{[5
]}

Skall, H. F. ^{[5
]}

Lee, P-Y ^{[6
]}

Zheng, S. ^{[2
]}

Monaghan, S. ^{[7
]}

Reiche, S. ^{[1
]}

Fuchs, W. ^{[1
]}

Kotler, M. ^{[8
]}

Way, K. ^{[9
]}

Braeuer, G. ^{[10
]}

Boettcher, K. ^{[10
]}

Kappe, A. ^{[11
]}

Kielpinska, J. ^{[12
]}

机构：

[1] Fed Res Inst Anim Hlth, Friedrich Loeffler Inst, Sudufer 10, D-17493 Greifswald, Germany

[2] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

[3] Natl Inst Vet Res, Dept Fish Dis, Pulawy, Poland

[4] Agence Natl Secur Sanit Alimentat Environm Travai, Ploufragan, France

[5] Tech Univ Denmark, Sect Diagnost & Sci Advice, Natl Vet Inst, Frederiksberg C, Denmark

[6] GeneReach Biotechnol Corp, Dept Res & Dev, Taichung, Taiwan

[7] Univ Stirling, Aquat Vaccine Unit, Sch Nat Sci, Inst Aquaculture, Stirling, Scotland

[8] Hebrew Univ Jerusalem, Dept Pathol, Hadassah Med Sch, Jerusalem, Israel

[9] Ctr Environm Fisheries & Aquaculture Sci, Weymouth, Dorset, England

[10] Fish Hlth Serv Saxony, Dresden, Germany

[11] Fish Hlth Serv Thuringia, Bad Langensalza, Germany

[12] West Pomeranian Univ Technol, Dept Fac Aquaculture Food Sci & Fisheries, Szczecin, Poland

来源：

JOURNAL OF FISH DISEASES | 2017年 / 40卷 / 11期

关键词：

carp and koi sera; enzyme-linked immunosorbent assay; koi herpesvirus; koi herpesvirus antibodies; KOI HERPESVIRUS KHV; CYPRINUS-CARPIO-KOI; COMMON CARP; STRUCTURAL PROTEINS; CHANNEL CATFISH; VIRUS; GENOME; FISH; AQUACULTURE; EMERGENCE;

D O I：

10.1111/jfd.12621

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

引用

页码：1511 / 1527

页数：17

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