Downregulated Expression of Virulence Factors Induced by Benzyl Isothiocyanate in Staphylococcus Aureus: A Transcriptomic Analysis

被引:12
|
作者
Wang, Xiaoning [1 ]
Wu, Hongyan [2 ]
Niu, Tongxin [1 ]
Bi, Jingran [1 ]
Hou, Hongman [1 ]
Hao, Hongshun [3 ]
Zhang, Gongliang [1 ]
机构
[1] Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
[2] Okayama Univ, Grad Sch Environm & Life Sci, Okayama 7008530, Japan
[3] Dalian Polytech Univ, Dept Inorgan Nonmetall Mat Engn, Dalian 116034, Peoples R China
基金
中国国家自然科学基金;
关键词
Staphylococcus aureus; benzyl isothiocyanate; RNA-seq; differentially expressed genes; biofilm; CAPSULAR POLYSACCHARIDE; ANTIBACTERIAL ACTIVITY; MECHANISM; RECOVERY; PCR;
D O I
10.3390/ijms20215441
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Staphylococcus aureus (S. aureus) is a common foodborne pathogen that leads to various diseases; therefore, we urgently need to identify different means to control this harmful pathogen in food. In this study, we monitored the transcriptional changes of S. aureus by RNA-seq analysis to better understand the effect of benzyl isothiocyanate (BITC) on the virulence inhibition of S. aureus and determined the bacteriostatic effect of BITC at subinhibitory concentrations. Our results revealed that, compared with the control group (SAC), the BITC-treated experimental group (SAQ_BITC) had 708 differentially expressed genes (DEGs), of which 333 genes were downregulated and the capsular polysaccharide (cp) was significantly downregulated. Furthermore, we screened five of the most virulent factors of S. aureus, including the capsular polysaccharide biosynthesis protein (cp5D), capsular polysaccharide synthesis enzyme (cp8F), thermonuclease (nuc), clumping factor (clf), and protein A (spa), and verified the accuracy of these significantly downregulated genes by qRT-PCR. At the same time, we used light microscopy, scanning electron microscopy (SEM) and inverted fluorescence microscopy (IFM) to observe changes in biofilm associated with the cp5D and cp8F. Therefore, these results will help to further study the basis of BITC for the antibacterial action of foodborne pathogenic bacteria.
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页数:13
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