Intracellular accumulation and immunological properties of fluorescent gold nanoclusters in human dendritic cells

被引:100
|
作者
Fernandez, Tahia D. [1 ]
Pearson, John R. [2 ]
Leal, Manuel Pernia [3 ]
Torres, Maria J. [1 ,4 ]
Blanca, Miguel [1 ,4 ]
Mayorga, Cristobalina [1 ,4 ]
Le Guevel, Xavier [5 ]
机构
[1] UMA, Reg Univ Malaga Hosp, IBIMA, Res Lab & Allergy Serv, Malaga, Spain
[2] Univ Malaga, Andalusian Ctr Nanomed & Biotechnol BIONAND, Nanoimaging Unit, E-29071 Malaga, Spain
[3] Univ Malaga, Andalusian Ctr Nanomed & Biotechnol BIONAND, Diagnost Area, E-29071 Malaga, Spain
[4] Carlos Haya Hosp, Allergy Serv, Malaga, Spain
[5] BIONAND, Andalusian Ctr Nanomed & Biotechnol, Malaga, Spain
关键词
Gold nanoclusters; Fluorescence; Cellular uptake; Immune response; Monocyte-derived dendritic cells; POLYETHYLENE-GLYCOL; QUANTUM DOTS; SILVER NANOPARTICLES; VACCINE ADJUVANTS; SIZE; STABILITY; INTERNALIZATION; MATURATION; COMPLEXES; RESPONSES;
D O I
10.1016/j.biomaterials.2014.11.045
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We have studied the effect of highly fluorescent gold nanoclusters (Au NCs) (0 < 3 nm) stabilized by different ligands on the intracellular accumulation and immune response of human derived-monocyte dendritic cells (DCs). Results indicate that the high uptake efficiency of Au NCs is strongly related to their small size and to the nature of the ligand, with zwitterionic ligands being more effective than PEGylated ones. Evidence from flow cytometry and microscopy demonstrate time and concentration-dependent Au NCs internalization by endocytic pathway(s) involving amorphous and laminar organelles, while maintaining their discrete size and photoluminescence properties. The uptake of zwitterionic ligand-stabilized Au NCs induced very low cytotoxicity and a strong immunosuppressive response (Thu/ Treg pattern), associated with a DC maturation state. This behavior contrasts to the effect of bigger particles (similar to 12 nm size) which induced a cytotoxic response involving Natural Killer (CD56) cells. Overall, this study stresses the critical importance of particle size and ligand type on the immunostimulation of DCs and highlights the remarkable potential of this new class of nanomaterial as a novel vaccine platform. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1 / 12
页数:12
相关论文
共 50 条
  • [1] Intracellular Thermometry by Using Fluorescent Gold Nanoclusters
    Shang, Li
    Stockmar, Florian
    Azadfar, Naghmeh
    Nienhaus, G. Ulrich
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (42) : 11154 - 11157
  • [2] Chromium (VI)-induced immunotoxicity and intracellular accumulation in human primary dendritic cells
    Burastero, S. E.
    Paolucci, C.
    Breda, D.
    Ponti, J.
    Munaro, B.
    Sabbioni, E.
    [J]. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2006, 19 (03) : 581 - 591
  • [3] Immunological properties of human lymphoid related dendritic cells generated in vitro
    Wesa, A
    Galy, A
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2000, 114 (01) : 228 - 228
  • [4] In situ Synthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells
    West, Abby L.
    Schaeublin, Nicole M.
    Griep, Mark H.
    Maurer-Gardner, Elizabeth I.
    Cole, Daniel P.
    Fakner, Alexis M.
    Hussain, Saber M.
    Karna, Shashi P.
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (33) : 21221 - 21227
  • [5] Uptake of Ultrasmall Fluorescent Gold Nanoclusters by Live Hela Cells
    Yang, Linxiao
    Shang, Li
    Nienhaus, G. Ulrich
    [J]. BIOPHYSICAL JOURNAL, 2013, 104 (02) : 161A - 161A
  • [6] Multivalent Glycosylation of Fluorescent Gold Nanoclusters Promotes Increased Human Dendritic Cell Targeting via Multiple Endocytic Pathways
    Le Guevel, Xavier
    Perez Perrino, Monica
    Fernandez, Tahia D.
    Palomares, Francisca
    Torres, Maria-Jose
    Blanca, Miguel
    Rojo, Javier
    Mayorga, Cristobalina
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (37) : 20945 - 20956
  • [7] The study of immunological properties of the human dendritic cells pulsed with HBV antigen in vitro
    Liu, Yuan
    Li, Jun
    Han, Ya-Ping
    Huang, Zu-Hu
    [J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2006, 21 : A132 - A132
  • [8] Targeting dendritic cells through gold nanoparticles: A review on the cellular uptake and subsequent immunological properties
    Ahmad, Suhana
    Zamry, Anes Ateqah
    Tan, Hern-Tze Tina
    Wong, Kah Keng
    Lim, JitKang
    Mohamud, Rohimah
    [J]. MOLECULAR IMMUNOLOGY, 2017, 91 : 123 - 133
  • [9] Fluorescent gold nanoclusters on/in cells visualized by fluorescence lifetime imaging microscopy
    Mutas, Marina
    Hadler, Tim
    Strelow, Christian
    Kipp, Tobias
    Mews, Alf
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 254
  • [10] INTRACELLULAR ACCUMULATION OF A FLUORESCENT DERIVATIVE OF PAROMOMYCIN IN HUMAN-FIBROBLASTS
    BUCHANAN, JH
    RATTAN, SIS
    STEVENS, A
    HOLLIDAY, R
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1982, 20 (01) : 71 - 80