Determination of phase II drug metabolites in equine urine by micellar electrokinetic capillary chromatography

被引：20
|
作者：
Taylor, MR [1 ]
Westwood, SA [1 ]
Perrett, D [1 ]
机构：
[1] UNIV LONDON ST BARTHOLOMEWS HOSP MED COLL,DEPT MED,LONDON EC1A 7BE,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 745卷 / 1-2期
关键词：
morphine; meclofenamic acid; glucuronides; non-steroidal anti-inflammatory drugs;
D O I：
10.1016/0021-9673(96)00380-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Micellar electrokinetic capillary chromatography (MECC) using diode array detection has been investigated for the determination of phase I and phase II metabolites of drugs in biofluids. Methods were optimised for the determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide, normorphine, meclofenamic acid and its metabolites in equine urine. Solid-phase extraction procedures were developed to concentrate and purify the analytes from spiked and post administration urines for MECC analysis. A simple on-line procedure for monitoring the kinetics of hydrolysis of morphine-glucuronide conjugates by P-glucuronidase was demonstrated.
引用
收藏
页码：155 / 163
页数：9
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